Proteomics analysis of latex from Hevea brasiliensis (clone RRIM 600)

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Proteomics analysis of latex from Hevea brasiliensis (clone RRIM 600).

The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Following ultracentrifugation, the latex can be separated into 3 layers: C-serum, lutoids, and rubber particles. Previous studies have shown that a large number of proteins are present in these 3 layers. However, a complete proteome for this important plant is still un...

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Allergenic Proteins of Hevea brasiliensis Latex Fractions

A spectrum of proteins derived from the rubber tree, Hevea brasiliensis, reacted with IgE in the plasma from persons allergic to natural rubber latex. Latex B-serum, C-serum and rubber particle membrane proteins were all found to contain allergenie proteins. Variations were observed in the reactivity of the latex, IgE positive plasma to the different proteins. Monoclonal antibodies have been de...

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Glycolipid composition of Hevea brasiliensis latex.

Glycolipids of fresh latex from three clones of Hevea brasiliensis were characterized and quantified by HPLC/ESI-MS. Their fatty acyl and sterol components were further confirmed by GC/MS after saponification. The four detected glycolipid classes were steryl glucosides (SG), esterified steryl glucosides (ESG), monogalactosyl diacylglycerols (MGDG) and digalactosyl diacylglycerols (DGDG). Sterol...

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CDNA library from the latex of Hevea brasiliensis

Latex from Hevea brasiliensis contains 30-50% (w/w) of natural rubber (cis-1,4-polyisoprene), the important raw material for many rubber industries. We have constructed a cDNA library from the latex of H. brasiliensis to investigate the expressed genes and molecular events in the latex. We analyzed 412 expressed sequence tags (ESTs). More than 90% of the EST clones showed homology to previously...

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A Stimulator Protein of Rubber Biosynthesis from Hevea brasiliensis Latex

A rubber biosynthesis stimulator protein (RBSP)from the C-serum of Hevea brasiliensis latex was purified and characterised. This proteinaceous factor stimulates the incorporation of isopentenyl diphosphate into rubber and thereby enhances the rate of rubber biosynthesis. The heat stable RBSP is shown to have a molecular weight of 13 068 Da by mass spectroscopy. Amino acid sequence of peptide fr...

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ژورنال

عنوان ژورنال: Biochemistry and Cell Biology

سال: 2017

ISSN: 0829-8211,1208-6002

DOI: 10.1139/bcb-2016-0144